Rabbit submandibular salivary gland replantation
Almansoori Akram A., Khentii Namuun, Hei Wei-Hong, 서나리, 이성호, 김성민, 이종호,
소속 상세정보
( Almansoori Akram A. ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
( Khentii Namuun ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
( Hei Wei-Hong ) - Wuhan University School and Hospital of Stomatology
서나리 ( Seo Na-Ri ) - Seoul National University Dental Research Institute
이성호 ( Lee Sung-Ho ) - Seoul National University Dental Research Institute
김성민 ( Kim Soung-Min ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
이종호 ( Lee Jong-Ho ) - Seoul National University School of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy.
Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, 99mTc scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation.
Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight variations from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini.
Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.
키워드
Rabbits; Submandibular gland; Replantation
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